Molecular inference in the colonization of cattle in Ecuador.

The aim of the present paper was to evaluate genetic diversity within and among Ecuadorian bovine breeds using microsatellite markers. The genetic identity and the exotic influences from taurine or zebuine cosmopolitan breeds on Macabea, Loja, Santa Elena, Manabí and Isla de Puná breeds were evaluated. Twenty-eight microsatellite markers were tested across 254 individuals belonging to the five Ecuadorian populations to investigate intra and inter population genetic diversity levels. Nineteen markers in common with a set of 1595 samples of 35 previously typed breeds were used to determine the potential origin of Ecuadorian bovine breeds and to identify and quantify their genetic relationships. The findings of FIS > FST (0.0814 > 0.0499), even in the context of low but significant FST values, may be indicative of the intrapopulation origin of the variability of allelic frequencies across populations. Conclusively, the study of genetic distances and population structure suggested the origin of Ecuadorian populations may likely stem from Southern Spanish breeds, with no significant recent influence from cosmopolitan Taurine or Zebuine breeds. These findings may provide a solid basis for the demonstration of an Ecuadorian breeds identity in the framework of the genetic conservation of the American creole populations.

Genetic diversity evolution of a sheep breed reintroduced after extinction: Tracing back Christopher Columbus' first imported sheep.

New World's hair sheep breeds may genetically stem from West African introgression into established ecotypes of Spanish descent presumably extinct in the XIX Century. However, present Canary non-wooled breeds have presumably regressively resulted from the absorption of primitive individuals through Venezuelan descendants. We studied genetic diversity, structure, and evolution of the Canary hair sheep since its reintroduction in the 1950s. Demographic and genetic variability were evaluated using ENDOG (v4.8). Effective population size based on individual inbreeding rate was around one third higher than when based on individual coancestry rate. Nei's distances and equivalent subpopulations number indicated a highly-structured population. Although genetic diversity loss since the founder generations could be considered small, narrower pedigree bottlenecks could result from intraflock breeding policies and excessive contribution of few ancestors. Long generation intervals could be considered when reducing inbreeding. Wright's fixation statistics indicated slight interflock inbreeding. Pedigree completeness suggested genetic parameters were reliable, hence controlling inbreeding negative effects, could indeed, be crucial preserving these animal resources, consolidating the population in the archipelago after reintroduction.

Sensory Preference and Professional Profile Affinity Definition of Endangered Native Breed Eggs Compared to Commercial Laying Lineages' Eggs.

This study aimed to compare Utrerana native hen eggs' sensory properties to Leghorn Lohmann LSL-Classic lineage's commercial and ecological eggs through free-choice profiling. Second, affine and non-affine profiles were defined using the information provided by professionally-instructed panelists on six sets (sensory attributes, diet habits, production context awareness, product consciousness, cuisine applicability and panelist attributes) using nonlinear canonical correlation analysis. Sixty-four instructed professional panelists rated 96 eggs on 39 variables comprising the above-mentioned sets. Observers reported a significantly higher appreciation (p > 0.05) towards yolk color, odor, flavor, texture, overall score, and whole and on plate broken egg visual value when Utrerana eggs were compared to the rest of categories. Professional Profile A (PPA), or egg non-affine profile, consumed less eggs and provided lower scores to sensory attributes than Professional Profile B (PPB), or affine profile. Additionally, PPB accounted for higher knowledge about the Utrerana breed and provided greater importance to a product's ecological and autochthonous nature. PPA was generally characterized by women under 20 years old with no higher studies, while PPB comprised 21-40 years old men with secondary studies. In conclusion, defining professional profiles enables correctly approaching market needs to improve the profitability of Utrerana eggs, meeting professional demands and educating non-affine profiles.

Non-Parametrical Canonical Analysis of Quality-Related Characteristics of Eggs of Different Varieties of Native Hens Compared to Laying Lineage.

The aim of the present study is to characterize the productive capability of Utrerana and to compare the relationships among parameters determining the internal and external quality of the egg, through canonical correlation analysis. A flock of 68 Utrerana hens and a control group of Leghorn hens (n = 17) were housed individually to allow individual identification of eggs and for the assessment of egg quality characteristics. Almost all variables showed differences when both breeds were compared, except for white height, yolk diameter, yolkL* and yolk pH (p > 0.05). Only minor diameter, white height, yolkL*, yolka*, and shell weight reported significant differences between laying age groups. White height, yolk color, and almost all yolk color coordinates were significantly different (p < 0.05) for period and month. Egg and white weight reached highest significantly different levels for the fourth and fifth time that the hens laid an egg. External quality-related traits are better predictors of internal quality-related traits than vice versa, enabling the implementation of an effective noninvasive method for internal quality determination and egg classification aimed at suiting the needs of consumers.

Risk factor meta-analysis and Bayesian estimation of genetic parameters and breeding values for hypersensibility to cutaneous habronematidosis in donkeys.

Cutaneous habronematidosis (CH) is a highly prevalent seasonally recurrent skin disease that affects donkeys as a result from the action of spirurid stomach worm larvae. Carrier flies mistakenly deposit these larvae on previous skin lesions or on the moisture of natural orifices, causing distress and inflicting relapsing wounds to the animals. First, we carried out a meta-analysis of the predisposing factors that could condition the development of CH in Andalusian donkeys. Second, basing on the empirical existence of an inter and intrafamilial variation previously addressed by owners, we isolated the genetic background behind the hypersensibility to this parasitological disease. To this aim, we designed a Bayesian linear model (BLM) to estimate the breeding values and genetic parameters for the hypersensibility to CH as a way to infer the potential selection suitability of this trait, seeking the improvement of donkey conservation programs. We studied the historical record of the cases of CH of 765 donkeys from 1984 to 2017. Fixed effects included birth year, birth season, sex, farm/owner, and husbandry system. Age was included as a linear and quadratic covariate. Although the effects of birth season and birth year were statistically non-significant (P > 0.05), their respective interactions with sex and farm/owner were statistically significant (P < 0.01), what translated into an increase of 40.5% in the specificity and of 0.6% of the sensibility of the model designed, when such interactions were included. Our BLM reported highly accurate genetic parameters as suggested by the low error of around 0.005, and the 95% credible interval for the heritability of ±0.0012. The CH hypersensibility heritability was 0.0346. The value of 0.1232 for additive genetic variance addresses a relatively low genetic variation in the Andalusian donkey breed. Our results suggest that farms managed under extensive husbandry conditions are the most protective ones against developing CH. Furthermore, these results provide evidence of the lack of repercussion of other factors such as age or sex. Potentially considering CH hypersensibility as a negative selection aimed goal in donkey breeding programs, may turn into a measure to improve animal welfare indirectly. However, the low heritability value makes it compulsory to control environmental factors to ensure the effectiveness of the breeding measures implemented to obtain individuals that may genetically be less prone to develop the condition.